Evidence of free-radical-induced damage in rabbit kidneys after simple hypothermic preservation and autotransplantation.

摘要: Rabbit kidneys were stored for 24 or 48 hr at 0 degree C after single-passage vascular flush with 30 ml of cold hypertonic citrate solution 0.9% isotonic sodium chloride solution. They then subjected to in vitro biochemical assay evidence free-radical damage immediately storage they had been orthotopically autotransplanted and reperfused blood vivo 60 min. Kidney homogenates incubated 37 degrees assayed fluorescent conjugated Schiff bases as indicators lipid peroxidation, well superoxide dismutase activity reduced oxidized glutathione. In flushed citrate, no peroxidation could be detected hr. However, reperfusion significantly more (P less than 0.01) was evident. Storage saline produced still higher levels whether not 0.001). base formation inversely proportional the glutathione measured. No changes detected. It is concluded that important during ischemia but most occurs 60-min transplantation.