Engineering characteristics of a single‐use stirred bioreactor at bench‐scale: The Mobius CellReady 3L bioreactor as a case study
作者： Stephan C. Kaiser , Regine Eibl , Dieter Eibl
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摘要: Stirred single-use bioreactors can be used as substitutes for their conventional counterparts made of glass or stainless steel in the development and production biopharmaceuticals wherever possible. Various studies have confirmed comparability cell growth well product quantity quality. However, information about engineering characteristics is still rare. This study focuses on stirred Mobius® CellReady 3L bioreactor. The main parameters typical operation conditions animal cultivations are presented first time. Numerical simulations with a commercial CFD package (Fluent 6.3) were accomplished to obtain data single- multi-phase fluid flow, power input, mixing time oxygen mass transfer. results, which compared from experiments literature, reveal suitability bioreactor expansion protein cultures. Furthermore, enable comparisons other reusable culture at bench-scale.
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