Large-Eddy Simulations of microcarrier exposure to potentially damaging eddies inside mini-bioreactors

摘要: Abstract Mechanically stirred vessels equipped with rotating impellers generate heterogeneous transitional or turbulent flows. However, some cells as animal human mesenchymal stem (hMSC) adhered on microcarriers, are reputed sensitive to hydromechanical stresses arising from stirring. Many publications, especially using Computational Fluid Dynamics, characterize spatial fields of velocity and turbulence inside bioreactors but the exposure frequency these is never taken into account in case cell culture bioreactor description. To fill this gap, study used both CFD Reynolds-Averaged Large-Eddy Simulations hydrodynamics 250 mL mini-bioreactors, which a relevant volume for hMSC cultures. Five impeller geometries were studied. From calculated, an energy dissipation/circulation function, related intensity potentially damaging hydrodynamic events cells, was determined various operating conditions. Based simulation results, marine propeller up-pumping mode seems be most adapted condition, since it exhibits low acceptable dissipation rate. general point view, new methodology proposed should future screen geometry biological constraints.