Analysis of the effect of LRP-1 silencing on the invasive potential of cancer cells by nanomechanical probing and adhesion force measurements using atomic force microscopy

摘要: Low-density lipoprotein receptor-related protein 1 (LRP-1) can internalize proteases involved in cancer progression and is thus considered a promising therapeutic target. However, it has been demonstrated that LRP-1 also able to regulate the endocytosis of membrane-anchored proteins. Thus, strategies target modulate proteolysis could affect adhesion cytoskeleton dynamics. Here, we investigated effect silencing on parameters reflecting cells’ invasiveness by atomic force microscopy (AFM). The results show induces changes behavior, particularly dynamics cell attachment. Clear alterations morphology, such as more pronounced stress fibers increased spreading, leading area circularity, were observed. determination mechanical properties AFM showed these differences are correlated with an increase Young's modulus. Moreover, measurements overall decrease motility modifications directional persistence. An between LRP-1-silenced cells gelatin-coated bead was Ultimately, our AFM-based spectroscopy data, recorded using antibody directed against β1 integrin subunit, provide evidence modifies rupture distribution. Together, techniques traditionally used for investigation be coupled gain access complementary phenotypic help discriminate specific phenotypes associated different degrees invasiveness.