Use of alternate splice sites in granule-bound starch synthase mRNA from low-amylose rice varieties

摘要: The rice Waxy gene encodes a granule-bound starch synthase (GBSS) necessary for the synthesis of amylose in endosperm tissue. We have previously shown that CT microsatellite near transcriptional start site GBSS can distinguish 7 alleles accounted more than 80% variation apparent content an extended pedigree 89 US cultivars (Oryza sativa L.). Furthermore, all with 18% or less were to sequence AGTTATA at putative leader intron 5′ splice site, while higher proportion had AGGTATA. Here we demonstrate this single-base mutation reduces efficiency pre-mRNA processing and results alternate splicing three cryptic sites. predominant CT18 low-amylose varieties is 93 bp upstream used intermediate high immediately demonstrated be tightly correlated content. Use either -93 -1 conjunction 3′ formation small open reading frame 38 normal ATG out it. This not produced when any sites are five bases further downstream which was observed tested.