The primary and secondary structure of yeast 26S rRNA

摘要: We present the sequence of 26S rRNA yeast Saccharomyces carlsbergensis as inferred from gene sequence. The molecule is 3393 nucleotides long and consists 48% G+C; 30 43 methyl groups can be located in Starting recently proposed structure E. coli 23S (see ref. 25) we constructed a secondary model for rRNA. This composed 7 domains closed by long-range base pairings n bacterial counterpart. Most show considerable conservation overall structure; unpaired regions extended homology base-paired contain many compensating pair changes. extra length due to number insertions most domains, particularly domain II. Domain VI, which extremely conserved, probably part ribosomal A site. alpha-Sarcin, apparently inhibits EF-1 dependent binding aminoacyl-tRNA, causes cleavage between position 3025 3026 conserved loop structure, just outside VI. Nearly all groups, like coli, are II, V VI clustered certain extent mainly with strongly primary structure. only three introduced relatively late during processing found single stranded loops very close positions have been shown at interface ribosome.