Specific solubilization of impurities in culture media: Arg solution improves purification of pH-responsive tag CspB50 with Teriparatide.

摘要: Abstract Protein purification using non-chromatographic methods is a simple technique that avoids costly resin. Recently, cell surface protein B (CspB) tag has been developed for pH-responsive by solid-liquid separation. Proteins fused with the CspB show reversible insolubilization at acidic pH can be used in separation purification. However, brown-color impurities from co-precipitation hamper further analysis of target proteins. In this study, we investigated effect additives on CspB-tagged Teriparatide (CspB50TEV-Teriparatide) expressed Corynebacterium glutamicum and associated impurities. Arginine (Arg) 1.0 M was found to most effective additive removing impurities, particularly carotenoids nucleic acids. Furthermore, all detected fluorescence absorbance spectra were successfully removed repetition precipitation-redissolution Arg solution. The precipitation yield CspB50TEV-Teriparatide did not change addition process. Collectively, our findings indicate specific desorption π-electron rich compounds may useful conjunction