Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains

作者: Fantao Kong , Tomohito Yamasaki , Sari Dewi Kurniasih , Liyuan Hou , Xiaobo Li

DOI: 10.1016/J.JBIOSC.2015.01.005

关键词:

摘要: … the Chlamydomonas squalene synthase (SQS) cDNA was much more efficiently expressed … Recently, using the Chlamydomonas MmeI-based insertion site Sequencing (ChlaMmeSeq) …

参考文章(42)
Pablo de Felipe, Skipping the co-expression problem: the new 2A "CHYSEL" technology. Genetic Vaccines and Therapy. ,vol. 2, pp. 13- 13 ,(2004) , 10.1186/1479-0556-2-13
Michael R. Rountree, Kurtis E. Bachman, Stephen B. Baylin, DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci Nature Genetics. ,vol. 25, pp. 269- 277 ,(2000) , 10.1038/77023
Kosuke Shimogawara, Arthur Grossman, Shoko Fujiwara, Hideaki Usuda, High-Efficiency Transformation of Chlamydomonas reinhardtii by Electroporation Genetics. ,vol. 148, pp. 1821- 1828 ,(1998) , 10.1093/GENETICS/148.4.1821
Beth A. Rasala, Machiko Muto, Philip A. Lee, Michal Jager, Rosa M.F. Cardoso, Craig A. Behnke, Peter Kirk, Craig A. Hokanson, Roberto Crea, Michael Mendez, Stephen P. Mayfield, Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii Plant Biotechnology Journal. ,vol. 8, pp. 719- 733 ,(2010) , 10.1111/J.1467-7652.2010.00503.X
David González-Ballester, Amaury de Montaigu, Aurora Galván, Emilio Fernández, Restriction enzyme site-directed amplification PCR: a tool to identify regions flanking a marker DNA. Analytical Biochemistry. ,vol. 340, pp. 330- 335 ,(2005) , 10.1016/J.AB.2005.01.031
Alke Eichler-Stahlberg, Wolfram Weisheit, Ovidiu Ruecker, Markus Heitzer, Strategies to facilitate transgene expression in Chlamydomonas reinhardtii Planta. ,vol. 229, pp. 873- 883 ,(2009) , 10.1007/S00425-008-0879-X
Beth A. Rasala, Philip A. Lee, Zhouxin Shen, Steven P. Briggs, Michael Mendez, Stephen P. Mayfield, Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide PLoS ONE. ,vol. 7, pp. e43349- ,(2012) , 10.1371/JOURNAL.PONE.0043349
D. S. Gorman, R. P. Levine, Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 54, pp. 1665- 1669 ,(1965) , 10.1073/PNAS.54.6.1665
Ovidiu Ruecker, Karina Zillner, Regina Groebner-Ferreira, Markus Heitzer, Gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii. Molecular Genetics and Genomics. ,vol. 280, pp. 153- 162 ,(2008) , 10.1007/S00438-008-0352-3