Green tea extract attenuates hepatic steatosis by decreasing adipose lipogenesis and enhancing hepatic antioxidant defenses in ob/ob mice

摘要: Excess hepatic lipid accumulation and oxidative stress contribute to nonalcoholic fatty liver disease (NAFLD). Thus, we hypothesized that the hypolipidemic antioxidant activities of green tea extract (GTE) would attenuate events leading NAFLD. Obese mice (ob/ob; 5 weeks old, n=38) their lean littermates (n=12) were fed 0%, 0.5% or 1% GTE for 6 weeks. Then, steatosis, inflammatory markers measured. mice, compared controls, had greater lipids serum alanine aminotransferase (ALT). at lowered (P<.05) ALT in obese mice. The GTE-mediated attenuation steatosis was accompanied by decreased mRNA expression adipose sterol regulatory element-binding protein-1c, acid synthase, stearoyl CoA desaturase-1, hormone-sensitive lipase nonesterified concentrations. Immunohistochemical data indicated steatotic livers from extensive tumor necrosis factor-α (TNF-α), whereas TNF-α protein inhibited expression. Hepatic total glutathione, malondialdehyde Mn- Cu/Zn-superoxide dismutase normalized levels littermates. Also, increased catalase glutathione peroxidase activities, these inversely correlated with lipids. Collectively, mitigated NAFLD injury ob/ob decreasing release acids inhibiting peroxidation as well restoring defenses responses. These findings suggest may be used an effective dietary strategy mitigate obesity-triggered