Intron-mediated RNA interference and microRNA (miRNA)

摘要: MicroRNAs (miRNAs) are small single-stranded regulatory RNAs capable of interfering with messenger (mRNAs) through complete or partial complementarities. Partial complementarity gives miRNAs a flexibility which is useful for construction new therapies against cancer polymorphisms and viral mutations. Varieties have been reported in diverse species; they believed to induce RNA interference (RNAi), post-transcriptional gene silencing mechanism. Recently, many intronic sequences shown encode microRNAs. Intronic miRNA, class miRNAs, derived from introns by splicing Dicer processing, it differs uniquely previously described intergenic miRNA that require type II polymerases (Pol-II) spliceosomal components their biogenesis. Several kinds identified; however, functions applications not reported. Here, we show the first time intron-derived able cells demonstrating evolutionary preservation this system vivo.