Inducable expression from the eukaryotic pol -2 promoter in prokaryotes
作者： Shi-Lung Lin , Donald C. Chang
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摘要: Eukaryotic protein-coding messenger RNAs and non-coding microRNAs are naturally transcribed by type II RNA polymerases (pol-2) but not prokaryotic polymerases. As a result, current eukaryotic protein production is performed either using pol-2 promoters in hybridomas or mammalian cells bacterial cells. However, because transcription tends to be error-prone, frequent mutation big problem. Also, growing relatively laborious costly. To overcome these problems, the present invention provides novel inducible composition method for producing and/or their related peptides/proteins directly promoter-driven gene expression fast bacteria, without need of changing hybridomas/mammalian The so obtained can used develop drugs, cure diseases, treat tumors/cancers, produce pluripotent stem (iPS) cells, enhance wound healing, make foods.
google.nl 参考文章(12)
Shi-Lung Lin, Shao-Yao Ying, Role of mir-302 MicroRNA Family in Stem Cell Pluripotency and Renewal Springer, Dordrecht. pp. 167- 185 ,(2008) , 10.1007/978-1-4020-8533-8_10
Jochen G. Salfeld, Manfred Gossen, Jeffrey W. Voss, Hermann Bujard, Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters ,(1994)
F. Pantanella, F. Berlutti, C. Passariello, S. Sarli, C. Morea, S. Schippa, Violacein and biofilm production in Janthinobacterium lividum. Journal of Applied Microbiology. ,vol. 102, pp. 992- 999 ,(2006) , 10.1111/J.1365-2672.2006.03155.X
Shi-Lung Lin, Intron-mediated RNA interference and microRNA (miRNA) Frontiers in Bioscience. ,vol. 13, pp. 2216- ,(2008) , 10.2741/2836
Stina Simonsson, John Gurdon, DNA demethylation is necessary for the epigenetic reprogramming of somatic cell nuclei Nature Cell Biology. ,vol. 6, pp. 984- 990 ,(2004) , 10.1038/NCB1176
Q. Gao, A. Fang, D. L. Pierson, S. K. Mishra, A. L. Demain, Shear stress enhances microcin B17 production in a rotating wall bioreactor, but ethanol stress does not. Applied Microbiology and Biotechnology. ,vol. 56, pp. 384- 387 ,(2001) , 10.1007/S002530100610
M. Gossen, H. Bujard, Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 5547- 5551 ,(1992) , 10.1073/PNAS.89.12.5547
Christopher P. Meenan, Martha V. L. Ray, Angelo P. Consalvo, Nozar M. Mehta, Direct expression of peptides into culture media ,(1998)
Shi-Lung Lin, Donald C. Chang, Shao-Yao Ying, Davey Leu, David T.S. Wu, MicroRNA miR-302 Inhibits the Tumorigenecity of Human Pluripotent Stem Cells by Coordinate Suppression of the CDK2 and CDK4/6 Cell Cycle Pathways Cancer Research. ,vol. 70, pp. 9473- 9482 ,(2010) , 10.1158/0008-5472.CAN-10-2746
S.-L. Lin, D. C. Chang, S. Chang-Lin, C.-H. Lin, D. T.S. Wu, D. T. Chen, S.-Y. Ying, Mir-302 reprograms human skin cancer cells into a pluripotent ES-cell-like state RNA. ,vol. 14, pp. 2115- 2124 ,(2008) , 10.1261/RNA.1162708