Direct expression of peptides into culture media

摘要: Expression systems are disclosed for the direct expression of peptide products into culture media where genetically engineered host cells grown. High yield was achieved with novel vectors, a special selection hosts, and/or fermentation processes which include careful control cell growth rate, and use an inducer during phase. Special vectors provided regions having multiple promoters linked operably coding encoding signal upstream from region interest. Multiple transcription cassettes also used to increase yield. The production amidated peptides using is disclosed.