Tyrosine phosphorylation of paxillin affects the metastatic potential of human osteosarcoma.

摘要: To acquire information on signal alteration corresponding to the changes in metastatic potential, we analysed protein tyrosine phosphorylation of low- and high-metastatic human osteosarcoma HuO9 sublines, which were recently established as first model osteosarcoma. Tyrosine proteins around 60, 70, 120-130 kDa was enhanced sublines. Among these proteins, 70 kDa, most remarkably phosphorylated, identified paxillin, a scaffold integrin signaling. Activity Src family kinase correlated well with inhibitor, PP2, not only abolished paxillin but also impaired motility The expression elevated knocking down by RNAi method resulted attenuated cells. We demonstrated that phosphorylated form is essential for migration-promoting effect These findings suggest activity kinases overexpression synergistically contribute high potential through hyperphosphorylation paxillin.