Protease sensing using nontoxic silicon quantum dots.

作者: Xiaoyu Cheng , Benjamin F. P. McVey , Andrew B. Robinson , Guillaume Longatte , Peter B. O’Mara

DOI: 10.1117/1.JBO.22.8.087002

关键词:

摘要: Herein is presented a proof-of-concept study of protease sensing that combines nontoxic silicon quantum dots (SiQDs) with Forster resonance energy transfer (FRET). The SiQDs serve as the donor and an organic dye acceptor. covalently attached to using peptide linker. Enzymatic cleavage leads changes in FRET efficiency. combination interfacial design optical imaging this work opens opportunities for use relevant intracellular imaging.

参考文章(34)
Chongxi Song, Yiling Zhong, Xiangxu Jiang, Fei Peng, Yimei Lu, Xiaoyuan Ji, Yuanyuan Su, Yao He, Peptide-Conjugated Fluorescent Silicon Nanoparticles Enabling Simultaneous Tracking and Specific Destruction of Cancer Cells Analytical Chemistry. ,vol. 87, pp. 6718- 6723 ,(2015) , 10.1021/ACS.ANALCHEM.5B00853
Sebastián A. Díaz, Anthony P. Malonoski, Kimihiro Susumu, Romina V. Hofele, Eunkeu Oh, Igor L. Medintz, Probing the kinetics of quantum dot-based proteolytic sensors Analytical and Bioanalytical Chemistry. ,vol. 407, pp. 7307- 7318 ,(2015) , 10.1007/S00216-015-8892-Y
Xiaoyu Cheng, Elizabeth Hinde, Dylan M. Owen, Stuart B. Lowe, Peter J. Reece, Katharina Gaus, J. Justin Gooding, Enhancing Quantum Dots for Bioimaging using Advanced Surface Chemistry and Advanced Optical Microscopy: Application to Silicon Quantum Dots (SiQDs). Advanced Materials. ,vol. 27, pp. 6144- 6150 ,(2015) , 10.1002/ADMA.201503223
Richard K. Baldwin, Katherine A. Pettigrew, Jayne C. Garno, Phillip P. Power, Gang-yu Liu, Susan M. Kauzlarich, Room temperature solution synthesis of alkyl-capped tetrahedral shaped silicon nanocrystals. Journal of the American Chemical Society. ,vol. 124, pp. 1150- 1151 ,(2002) , 10.1021/JA017170B
Folarin Erogbogbo, Ching-Wen Chang, Jasmine May, Paras N. Prasad, Mark T. Swihart, Energy transfer from a dye donor to enhance the luminescence of silicon quantum dots. Nanoscale. ,vol. 4, pp. 5163- 5168 ,(2012) , 10.1039/C2NR31003A
Amane Shiohara, Sanshiro Hanada, Sujay Prabakar, Kohki Fujioka, Teck H. Lim, Kenji Yamamoto, Peter T. Northcote, Richard D. Tilley, Chemical Reactions on Surface Molecules Attached to Silicon Quantum Dots Journal of the American Chemical Society. ,vol. 132, pp. 248- 253 ,(2010) , 10.1021/JA906501V
Allison M. Dennis, Won Jong Rhee, David Sotto, Steven N. Dublin, Gang Bao, Quantum Dot-Fluorescent Protein FRET Probes for Sensing Intracellular pH ACS Nano. ,vol. 6, pp. 2917- 2924 ,(2012) , 10.1021/NN2038077
Richard D. Tilley, Jamie H. Warner, Kazushige Yamamoto, Isao Matsui, Hiroyuki Fujimori, Micro-emulsion synthesis of monodisperse surface stabilized silicon nanocrystals Chemical Communications. pp. 1833- 1835 ,(2005) , 10.1039/B416069J
Fengjun Hua, Mark T. Swihart, Eli Ruckenstein, Efficient surface grafting of luminescent silicon quantum dots by photoinitiated hydrosilylation. Langmuir. ,vol. 21, pp. 6054- 6062 ,(2005) , 10.1021/LA0509394