Peptic and tryptic digestion of peptides and proteins monitored by capillary electrophoresis with contactless conductivity detection.
作者： Aiping Schuchert-Shi , Peter C. Hauser
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摘要: The feasibility of monitoring the peptic and tryptic digestion peptides proteins with capillary electrophoresis using contactless conductivity detection was investigated. peptide minigastrin I cytochrome c from bovine heart, human serum albumin (HSA), myoglobin, (BSA) were digested off-line pepsin, resulting amino acid fragments successfully separated detected by measurement. Cytochrome myoglobin also subjected to cleavage trypsin. On-line electrophoretically mediated microanalysis (EMMA) approach demonstrated apomyoglobin
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