Monitoring of enzymatic reactions using conventional and on-chip capillary electrophoresis with contactless conductivity detection.
作者： Aiping Schuchert-Shi , Pavel Kubáň , Peter C. Hauser
关键词:
摘要: The use of CE with contactless conductivity detection was evaluated for monitoring enzymatic reactions. nonionic species ethanol, glucose, ethyl acetate, and butyrate were made accessible analysis by via charged products or by-products obtained in conversions using hexokinase, glucose oxidase, alcohol dehydrogenase, esterase. Two the reactions, namely conversion oxidase that ethylacetate esterase, also successfully demonstrated on a microchip device. Quantification taken as an example, found possible limit approximately 7 microM.
sci-hub.se 参考文章(30)
Pavel Kubáň, Peter C. Hauser, Contactless Conductivity Detection in Capillary Electrophoresis: A Review Electroanalysis. ,vol. 16, pp. 2009- 2021 ,(2004) , 10.1002/ELAN.200403125
Martina Petsch, Bernhard X. Mayer-Helm, Robert Sauermann, Christian Joukhadar, Ernst Kenndler, Capillary electrophoresis analysis of fosfomycin in biological fluids for clinical pharmacokinetic studies ELECTROPHORESIS. ,vol. 25, pp. 2292- 2298 ,(2004) , 10.1002/ELPS.200305860
Ling Zhang, Shokooh S Khaloo, Pavel Kubán, Peter C Hauser, Analysis of electroplating baths by capillary electrophoresis with high voltage contactless conductivity detection Measurement Science and Technology. ,vol. 17, pp. 3317- 3322 ,(2006) , 10.1088/0957-0233/17/12/018
Erik Baltussen, Rosanne M. Guijt, Gert van der Steen, Frederic Laugere, Sander Baltussen, Gijs W. K. van Dedem, Considerations on contactless conductivity detection in capillary electrophoresis Electrophoresis. ,vol. 23, pp. 2888- 2893 ,(2002) , 10.1002/1522-2683(200209)23:17<2888::AID-ELPS2888>3.0.CO;2-4
Jatisai Tanyanyiwa, Eva M. Abad-Villar, Peter C. Hauser, Contactless conductivity detection of selected organic ions in on-chip electrophoresis. Electrophoresis. ,vol. 25, pp. 903- 908 ,(2004) , 10.1002/ELPS.200305732
José Geraldo Alves Brito-Neto, José Alberto Fracassi da Silva, Lucas Blanes, Claudimir Lucio do Lago, Understanding Capacitively Coupled Contactless Conductivity Detection in Capillary and Microchip Electrophoresis. Part 1. Fundamentals Electroanalysis. ,vol. 17, pp. 1198- 1206 ,(2005) , 10.1002/ELAN.200503237
Jatisai Tanyanyiwa, Sandro Leuthardt, Peter C Hauser, Electrophoretic separations with polyether ether ketone capillaries and capacitively coupled contactless conductivity detection Journal of Chromatography A. ,vol. 978, pp. 205- 211 ,(2002) , 10.1016/S0021-9673(02)01425-5
Eva M. Abad-Villar, Jatisai Tanyanyiwa, M. Teresa Fernández-Abedul, Agustín Costa-García, Peter C. Hauser, Detection of human immunoglobulin in microchip and conventional capillary electrophoresis with contactless conductivity measurements Analytical Chemistry. ,vol. 76, pp. 1282- 1288 ,(2004) , 10.1021/AC0346656
Qi Jin Wan, Pavel Kubáň, Jatisai Tanyanyiwa, Andrea Rainelli, Peter C. Hauser, Determination of major inorganic ions in blood serum and urine by capillary electrophoresis with contactless conductivity detection Analytica Chimica Acta. ,vol. 525, pp. 11- 16 ,(2004) , 10.1016/J.ACA.2004.08.002
Pavel Kubáň, Peter C. Hauser, Effects of the cell geometry and operating parameters on the performance of an external contactless conductivity detector for microchip electrophoresis Lab Chip. ,vol. 5, pp. 407- 415 ,(2005) , 10.1039/B418845D