Following the lipase catalyzed enantioselective hydrolysis of amino acid esters with capillary electrophoresis using contactless conductivity detection

摘要: The progress of the enzymatic hydrolysis racemic mixtures enantiomers methyl esters serine and threonine was monitored. This possible in a reaction vessel 1.5 mL by direct sampling volumes nanoliter-range directly into an electrophoresis capillary. Contactless conductivity detection used for quantification as analytes are not accessible UV-detection capillary electrophoresis. Porcine pancreatic lipase wheat germ both showed preference L-enantiomers amino acid esters. selectivity porcine between two L-esters acids also studied it found that production L-threonine had priority over L-serine. Chirality, 2010. © 2009 Wiley-Liss, Inc.