Semi-automated radioimmunoassays for total serum thyroxine and triiodothyronine
作者： G.S. Challand , W.A. Ratcliffe , J.G. Ratcliffe
DOI: 10.1016/0009-8981(75)90176-X
关键词:
摘要: Single stage semi-automated radioimmunoassays for total serum thyroxine (T4) and triiodothyronine (T3) are described which employ an automatic pipetting station, gamma counter, a programmable calculator with paper tape reader printing facility. Both assays require only small volume of unextracted serum, specific sensitive. Their sample capacity, precision, speed, cost comparable the measurement protein-bound iodine. therefore have significant advantages over previous methods assessment thyroid function in diagnostic laboratory. A Simple method automating calculation results is described, applicable to any radioimmunoassay standard curve approximately linear on plot free/bound fraction against antigen concentration. In addition, general reported reveals relative contributions intrinsic, systematic, random error precision.
elsevier.com
sci-hub.se 参考文章(18)
Christine B Sekadde, W Roy Slaunwhite, Thomas Aceto, Rapid Radioimmunoassay of Triiodothyronine Clinical Chemistry. ,vol. 19, pp. 1016- 1021 ,(1973) , 10.1093/CLINCHEM/19.9.1016
T. Mitsuma, J. Colucci, L. Shenkman, C.S. Hollander, Rapid simultaneous radioimmunoassay for triiodothyronine and thyroxine in unextracted serum. Biochemical and Biophysical Research Communications. ,vol. 46, pp. 2107- 2113 ,(1972) , 10.1016/0006-291X(72)90766-8
W. A. RATCLIFFE, J. G. RATCLIFFE, A. D. McBRIDE, W. A. HARLAND, T. W. RANDALL, The radioimmunoassay of thyroxine in unextracted human serum. Clinical Endocrinology. ,vol. 3, pp. 481- 488 ,(1974) , 10.1111/J.1365-2265.1974.TB02819.X
BEVERLEY E. PEARSON MURPHY, CHAUNCEY J. PATTEE, DETERMINATION OF THYROXINE UTILIZING THE PROPERTY OF PROTEIN-BINDING. The Journal of Clinical Endocrinology and Metabolism. ,vol. 24, pp. 187- 196 ,(1964) , 10.1210/JCEM-24-2-187
A. T. Letchworth, R. J. Boardman, Christine Bristow, J. Landon, T. Chard, A RAPID SEMI-AUTOMATED METHOD FOR THE MEASUREMENT OF HUMAN CHORTONIC SOMATOMAMMOTROPHIN. THE NORMAL RANGE IN THE THIRD TRIMESTER AND ITS RELATION TO FETAL WEIGHT BJOG: An International Journal of Obstetrics and Gynaecology. ,vol. 78, pp. 542- 548 ,(1971) , 10.1111/J.1471-0528.1971.TB00313.X
R.P. Ekins, E.S. Williams, Ellis Sheila, The sensitive and precise measurement of serum thyroxine by saturation analysis (Competitive protein binding assay) Clinical Biochemistry. ,vol. 2, pp. 253- 288 ,(1968) , 10.1016/S0009-9120(68)80083-9
HOSSEIN GHARIB, W. E. MAYBERRY, ROBERT J. RYAN, RADIOIMMUNOASSAY FOR TRIIODOTHYRONINE: A PRELIMINARY REPORT The Journal of Clinical Endocrinology and Metabolism. ,vol. 31, pp. 709- 712 ,(1970) , 10.1210/JCEM-31-6-709
P. R. LARSEN, J. DOCKALOVA, D. SIPULA, F.M. WU, Immunoassay of thyroxine in unextracted human serum. The Journal of Clinical Endocrinology and Metabolism. ,vol. 37, pp. 177- 182 ,(1973) , 10.1210/JCEM-37-2-177
INDER J. CHOPRA, A Radioimmunoassay for Measurement of Thyroxine in Unextracted Serum The Journal of Clinical Endocrinology and Metabolism. ,vol. 34, pp. 938- 947 ,(1972) , 10.1210/JCEM-34-6-938
Inder J. Chopra, David H. Solomon, Gildon N. Beall, Radioimmunassay for measurement of triiodothyronine in human serum Journal of Clinical Investigation. ,vol. 50, pp. 2033- 2041 ,(1971) , 10.1172/JCI106696