Deactivation of linking moieties in antibody-enzyme conjugates

摘要: In some embodiments, the present invention pertains to a method for conjugating first compound second wherein conjugation involves an electrophilic moiety. The comprises reacting with form conjugate. improvement in embodiments of adding nucleophilic reagent conjugate forms neutral product upon reaction unreacted moieties is substantially non-reactive disulfide bonds event that bonds. formed doubly deactivated because other moiety linking also deactivated.