Preparation of an antibody-enzyme conjugate

摘要: An antibody-enzyme conjugate is prepared having an enzyme to antibody ratio of approximately 3. The produced by adding sulfhydryl groups and maleimidyl produce a modified enzyme, reacting the conjugate. In producing about 15 molar excess reagents for introducing used. When four used, stopped after specified period time addition selective reagents. may be cysteine iodoacetamide.