Preparation of sized populations of liposomes

摘要: The present invention provides novel methods for generating aqueous liquid encapsulating multi-lamellar lipid vesicles (liposomes) wherein one or more lipids, conjugates, and an to be encapsulated, are agitated in a vessel the presence of spherical contact masses having mean diameter less than 3,000 microns, preferred size range being 50-100 microns diameter, resulting substantially homogeneous population diameters about 150 nanometers. allow use small quantities marker lipid, leave no residual solvents, only with glass surfaces, involve transfer liposome preparations from film drying vessels sizing apparatus.