LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin-embedded tissues.
作者： E Stathopoulos , G S Naeve , C R Taylor , A L Epstein
DOI: 10.1177/37.9.2671152
关键词:
摘要: We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with wide spectrum of sarcomas and normal cells mesenchymal derivation. However, unlike similar reagents, unreactive malignant lymphoid therefore displays more restricted immunoreactivity. Because ability stain routinely processed pathological tissues marked reactivity sarcomas, is unique reagent for the immunohistochemical diagnosis cancer.
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sci-hub.se 参考文章(31)
Warnaar So, Ruiter Dj, Schlingemann Ro, Blok J, Emeis Jj, Dingjan Gm, Monoclonal antibody PAL-E specific for endothelium. Laboratory Investigation. ,vol. 52, pp. 71- 76 ,(1985)
T. E. W. Feltkamp, Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. Immunology. ,vol. 18, pp. 875- ,(1970)
K. Dellagi, W. Vainchenker, G. Vinci, D. Paulin, J.C. Brouet, Alteration of vimentin intermediate filament expression during differentiation of human hemopoietic cells. The EMBO Journal. ,vol. 2, pp. 1509- 1514 ,(1983) , 10.1002/J.1460-2075.1983.TB01615.X
A. M. Gown, A. M. Vogel, Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. American Journal of Pathology. ,vol. 114, pp. 309- 321 ,(1984)
WM Parks, RD Gingrich, CE Dahle, JC Hoak, Identification and characterization of an endothelial, cell-specific antigen with a monoclonal antibody. Blood. ,vol. 66, pp. 816- 823 ,(1985) , 10.1182/BLOOD.V66.4.816.816
K Weber, M Osborn, Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology Laboratory Investigation. ,vol. 48, pp. 372- 394 ,(1983)
Allen M. Gown, Arthur M. Vogel, Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumors. American Journal of Clinical Pathology. ,vol. 84, pp. 413- 424 ,(1985) , 10.1093/AJCP/84.4.413
JN Winter, D Variakojis, AL Epstein, Phenotypic analysis of established diffuse histiocytic lymphoma cell lines utilizing monoclonal antibodies and cytochemical techniques Blood. ,vol. 63, pp. 140- 146 ,(1984) , 10.1182/BLOOD.V63.1.140.140
Jane Norma Winter, A. L. Epstein, R. I. Fox, R. J. Marder, Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. Journal of Immunology. ,vol. 133, pp. 1028- 1036 ,(1984)
Alan L. Epstein, Henry S. Kaplan, Feeder Layer and Nutritional Requirements for the Establishment and Cloning of Human Malignant Lymphoma Cell Lines Cancer Research. ,vol. 39, pp. 1748- 1759 ,(1979)