Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors.

摘要: Two monoclonal antibodies (LN-1, LN-2) reactive with B lymphocytes in B5 formalin-fixed, paraffin-embedded tissue sections have been produced by utilizing cell extracts from pokeweed mitogen-stimulated peripheral blood and diffuse histiocytic lymphoma SU-DHL-4 cells, respectively. Both were initially identified indirect immunofluorescence screening techniques on paraformaldehyde-acetone-fixed preparations. Specificity screens 36 well-characterized human leukemia lines showed that both LN-1 LN-2 stained of lineage but unreactive those T or, one exception, myeloid derivation. Null acute lymphoblastic found to be LN-2+ LN-1-. The specificity these reagents was confirmed 15 17 biopsy specimens using techniques. Immunoperoxidase staining B5-fixed, lymphoid tissues bound the membrane cytoplasm germinal center cells whereas nuclear mantle zone as well interfollicular histiocytes thymic medullary dendritic cells. failed stain cortical thymocytes, lymph node 20 nonlymphoid organs revealed reacted positively red precursors bone marrow, ciliated epithelial bronchus, distal tubular kidney, ductal several including breast prostate. In contrast, all marrow. Indirect a panel 26 solid tumor majority epithelium-derived lines, glioblastomas, astrocytomas neuroblastomas, small carcinoma lung, sarcomas. 25 Immunobiochemical studies shown recognizes surface sialoantigen is directed against 35,000 dalton protein. Because their high for tumors ability tissues, are useful diagnosis classification lymphomas leukemias.