EVALUATION OF THE TWO-STAGE TECHNIQUE FOR THE IN VITRO ESTIMATION OF THE DRY MATTER DIGESTIBILITY OF CORN SILAGE

摘要: The two-stage Tilley and Terry technique (incubation with rumen fluid followed by an acid-pepsin digest), used to estimate dry matter (DM) digestibility of forages in vitro, was evaluated oven-dried corn silage as a substrate. effect weight substrate (100–3000 mg), continuous shaking incubations for the period incubation fluid, number bacteria present inoculum, contribution bacterial residual feed DM measured. Percent digested decreased linearly per tube increased. Continuous shaking, opposed intermittent mixing (twice daily) during increased rate disappearance resulted higher coefficients. Both volume inoculum that influenced percent digested. Bacteria contribute unless steps are taken remove them centrifugation or solubili...