Integrated analysis of microRNA and mRNA expression profiles highlights aldehyde-induced inflammatory responses in cells relevant for lung toxicity.

摘要: We have shown that aldehydes impact the bronchial airway gene expression associated with inflammatory responses. In this study, we sought to determine whether microRNA (miRNA) plays a role in regulating response aldehyde exposure. analyzed whole genome miRNA and mRNA profiles of human alveolar epithelial cells exposed 3 (propanal, butanal, pentanal) identify aldehyde-sensitive miRNAs characterize relationships between candidate cytokine-related genes, which are activated signals. Microarray analysis identified 15 for propanal, 25 10 pentanal, were differentially expressed A549 compared vehicle control samples. Integrated analyses significant miRNA-mRNA correlations. Gene ontology (GO) putative target genes (443 2166 364 showed biological category "cytokine-cytokine receptor interaction" was prominently annotated. Moreover, detected increased levels interleukin (IL)-6 IL-8 released exposure groups. Through an integrated aldehydes, provide evidence can affect cytokine-induced toxicity signaling. Therefore, study demonstrates added value approach identifying molecular events altered by environmental pollutants vitro model.