Allelotype analysis of childhood acute lymphoblastic leukemia.

摘要: Abstract To identify the genetic events that may play an important role in leukemogenesis of childhood ALL, we report for first time allelotyping ALL. Twenty-four cases ALL were screened loss heterozygosity (LOH) using 101 highly polymorphic microsatellite markers, which are distributed among all autosomal chromosomes. For LOH analysis on both chromosomes 9 and 12, 54 samples examined. The most frequent allelic was found chromosomal arm 9p, where 20 50 (40%) informative showed LOH. Moreover, nearly 30% did not have either homozygous deletions or point mutations putative tumor suppressor genes CDKN2/INK4A/p16 INK4B/p15 9p had at D9S171 . Loss 12p also (26%). Mutational suggested altered gene is cyclin-dependent kinase inhibitor p27/Kip1 , 12p. Several other regions included 1p, 4q, 5p, 6q, 7p, 8p, 9q, 10q, 13q, 17p, 17q, 18q, 19q, 22q. Of 24 patients, 19 (79%) least one arm. Samples two patients (8%) almost Fractional loss, calculated each sample as total number arms lost/total with information, a median value 0.04 mean 0.123 (range, 0 to 0.95). This fractional lower than those reported many solid tumors. shows extreme power markers