An arterial-specific enhancer of the human endothelin converting enzyme 1 (ECE1) gene is synergistically activated by Sox17, FoxC2, and Etv2.

摘要: Endothelin-converting enzyme-1 (Ece-1), a crucial component of the Endothelin signaling pathway, is required for embryonic development and an important regulator vascular tone, yet transcriptional regulation ECE1 gene has remained largely unknown. Here, we define activity enhancer from human locus in vivo. The identified here becomes active endothelial progenitor cells shortly after their initial specification dependent on conserved FOX:ETS motif, composite binding site Forkhead transcription factors Ets factor Etv2, motif bound cooperatively activated by FoxC2 but unlike other described FOX:ETS-dependent enhancers, restricted to arterial endothelium endocardium day 9.5 transgenic mouse embryos. also contains evolutionarily-conserved, consensus SOX site, which Importantly, Sox17, involved cell differentiation identity. Moreover, combinatorial action FoxC2, Sox17. Although Sox17 identity, few direct targets have been cells. Thus, this work implications our understanding subspecification.