Identification of bovine leukocyte antigen class II haplotypes associated with variations in bovine leukemia virus proviral load in Japanese Black cattle

摘要: Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which most common neoplastic disease cattle. leukocyte antigen (BoLA) strongly involved in subclinical progression BLV infections. Recent studies show that BoLA-DRB3 gene might play a direct role controlling number BLV-infected peripheral B lymphocytes vivo Holstein However, specific BoLA class II allele and DRB3-DQA1 haplotypes determining proviral load Japanese Black cattle are yet to be identified. In this study, we focused on association polymorphism We genotyped 186 BLV-infected, clinically normal for BoLA-DQA1 using polymerase chain reaction-sequence-based typing method. BoLA-DRB3*0902 BoLA-DRB3*1101 were associated with low (LPVL), BoLA-DRB3*1601 was high (HPVL). Furthermore, BoLA-DQA1*0204 BoLA-DQA1*10012 related LPVL HPVL, respectively. confirmed correlation between haplotype load. Two haplotypes, namely 0902B or C (DRB3*0902-DQA1*0204) 1101A (DRB3*1101-DQA1*10011), load, whereas one 1601B (DRB3*1601-DQA1*10012) conclude resistance dominant trait susceptibility recessive trait. Additionally, resistant alleles cattle, susceptible differed. This first report identify an variations