Expression of fibronectin, laminin and ribosomes in normal and nocodazole-treated neonatal heart cells in culture: a study by laser scanning confocal microscopy and immunocytochemistry.

摘要: Polyclonal and monoclonal antibodies were used to examine the effects of synthetic microtubule disruptive drug nocodazole on subcellular expression fibronectin, laminin, ribosomes in primary cultures neonatal cardiac ventricular cells. Non-invasive serial optical sectioning was carried out by immunolaser scanning confocal microscopy. In addition, fibronectin laminin immunolabelled with peroxidase or gold conjugates for electron-microscopic examination. Immunolabelling large 60S ribosome subunit fibroblast-like non-myocytes showed that punctate structures a multi-subunit composition present perinuclear region. Double immunostaining directed against cellular indicated cisternae rough endoplasmic reticulum. No clear treatment detected distribution cytoskeleton-bound ribosomes. Following immunolabelling both glycoproteins double 60 S subunit, found reticulum pleomorphic secretory vesicles. The cisternal stacks Golgi complex appeared either unstained only weakly labelled. When these cells exposed nocodazole, accumulated peripheral parts cytoplasm, including processes. These accumulations did not reflect staining, as shown labelling experiments versus wheat-germ-agglutinin and, exposing high dose brefeldin A.