Determination of methionine-enkephalin and leucine-enkephalin by LC-MS in human plasma: Study of pre-analytical stability.
作者： Aliye Ozalp , Begona Barroso , John Meijer , Cas van den Beld
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摘要: Abstract The aim of this work was to assess the influence preanalytical variables on stability two endogenous opioid peptides (Methionine-Enkephalin and Leucine-Enkephalin) in human plasma. For purpose, first a sensitive LC-MS/MS analytical method developed validated for simultaneous quantitative analysis these peptides. methodology consisted simple protein precipitation step followed by UPLC separation MRM using stable isotope labelled Methionine-Enkephalin as internal standard. with limit quantitation 10 pg/mL showed good reproducibility excellent accuracy precision, linear up 2000 pg/mL. An extensive evaluation pre-analytical blood carried out ensure an adequate sample collection procedure obtain reliable results clinical samples.
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