An improved method for determination of urinary 17-ketosteroids.

摘要: IN THE determination of urinary 17-ketosteroids, it has been recognized that interfering chromogens may cause significant errors. Three methods have introduced to eliminate or reduce these Talbot et al. (1) shown chromogenic materials are largely in the nonketonic fraction crude extract. The use Girard's reagent T results a chemical separation which eliminates this fraction, but is time-consuming procedure. correction equation involving aqueous alkali by Engstrom and Mason (2) introduce some serious Since there appears be no constant relationship between amounts 17-keto steroids substances, also qualitative differences not evident absorption maxima at wavelengths used various equations, indicating such rigid formula applicable every case. Cahen Salter (3) relatively simple method extracting final pink Zimmermann color with chloroform, undesirable pigments then remaining layer. chloroform was objectionable because turbidity encountered. (4) recently reported utilizing ether extraction color. Using identical samples, their values were close agreement those obtained on ketonic separated aid reagent. Their emphasized unreliability formulae. This paper describes for estimation total neutral utilizes amyl acetate as solvent