31P NMR Spectroscopy of the Mouse Heart

摘要: NMR spectroscopy of tissues and organs yields two kind information: the steady state tissue content abundant NMRobservable metabolites ions kinetics certain enzymes metabolic pathways. Because these measurements are noninvasive, they can be made repetitively throughout a protocol. They also simultaneously with assessment physiologic function. In case for 31P isolated heart, heart in situ, it is possible to make ATP phosphocreatine (PCr) contents turnover rates while assessing systolic diastolic performance. Such information unique provides valuable solution perennial conflict between biochemistry physiology. The biochemist dares extrapolate results obtained from dilute solutions protein-rich cytosol, hoping that have functional meaning. physiologist so convinced importance studying intact he/she treats as black-box about which no molecular known. biophysicist has best both worlds: using spectroscopy, one learn biochemical events within cell respecting integrity physiological system.