Comet assay reveals no genotoxicity risk of cationic solid lipid nanoparticles
作者： Slavomira Doktorovova , Amélia M Silva , Isabel Gaivão , Eliana B Souto , João P Teixeira
DOI: 10.1002/JAT.2961
关键词:
摘要: Cationic solid lipid nanoparticles (cSLN) are colloidal carriers for genes or drugs, particularly lipophilic drugs. Several reports exist on their high efficiency, but only a few studies report the effect of cSLNs living cells. In present work, internalization, cell viability (alamar blue assay) and genotoxic potential (alkaline comet three cSLN formulations (A–C) were evaluated in HepG2 Caco-2 showed an average hydrodynamic diameter (z-ave) 141–222 nm, zeta-potential 55.0–72.5 mV polidispersity indices (PdI) 0.336–0.421. Dispersion physiological buffers increased z-ave PdI. 0.01 mg ml–1 unaffected viability, 1.0 mg ml–1 significantly decreased it, being cSLN-C (Compritol-based) most toxic affected. DNA damage was not by 0.1 mg ml–1 observed at cSLN-C. Thus, no genotoxicity is to be expected concentrations that do reduce viability. Copyright © 2013 John Wiley & Sons, Ltd.
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sci-hub.se 参考文章(40)
Rainer H. Müller, Dörte Rühl, Stephan Runge, Kai Schulze‐Forster, Wolfgang Mehnert, Cytotoxicity of Solid Lipid Nanoparticles as a Function of the Lipid Matrix and the Surfactant Pharmaceutical Research. ,vol. 14, pp. 458- 462 ,(1997) , 10.1023/A:1012043315093
F. Alan Andersen, Safety assessment of cosmetic ingredients International Journal of Toxicology. ,vol. 26, pp. 1- 117 ,(2007)
Wesley F. Obritsch, Dale S. Gregerson, Donald J. Doughman, Eric M. Larson, A New, Simple, Nonradioactive, Nontoxic In Vitro Assay to Monitor Corneal Endothelial Cell Viability Investigative Ophthalmology & Visual Science. ,vol. 38, pp. 1929- 1933 ,(1997)
Clifford A. Barnes, Andreas Elsaesser, Joanna Arkusz, Anna Smok, Jadwiga Palus, Anna Leśniak, Anna Salvati, John P. Hanrahan, Wim H. de Jong, Elżbieta Dziubałtowska, Maciej Stȩpnik, Konrad Rydzyński, George McKerr, Iseult Lynch, Kenneth A. Dawson, C. Vyvyan Howard, Reproducible Comet Assay of Amorphous Silica Nanoparticles Detects No Genotoxicity Nano Letters. ,vol. 8, pp. 3069- 3074 ,(2008) , 10.1021/NL801661W
Ali Seyfoddin, John Shaw, Raida Al-Kassas, Solid lipid nanoparticles for ocular drug delivery Drug Delivery. ,vol. 17, pp. 467- 489 ,(2010) , 10.3109/10717544.2010.483257
Ashay Jain, Abhinav Agarwal, Saikat Majumder, Narendra Lariya, Anil Khaya, Himanshu Agrawal, Subrata Majumdar, Govind P. Agrawal, Mannosylated solid lipid nanoparticles as vectors for site-specific delivery of an anti-cancer drug Journal of Controlled Release. ,vol. 148, pp. 359- 367 ,(2010) , 10.1016/J.JCONREL.2010.09.003
S. Martins, S. Costa-Lima, T. Carneiro, A. Cordeiro-da-Silva, E.B. Souto, D.C. Ferreira, Solid lipid nanoparticles as intracellular drug transporters: an investigation of the uptake mechanism and pathway. International Journal of Pharmaceutics. ,vol. 430, pp. 216- 227 ,(2012) , 10.1016/J.IJPHARM.2012.03.032
Ritesh K. Shukla, Vyom Sharma, Alok K. Pandey, Shashi Singh, Sarwat Sultana, Alok Dhawan, ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells Toxicology in Vitro. ,vol. 25, pp. 231- 241 ,(2011) , 10.1016/J.TIV.2010.11.008
Ha Ryong Kim, Mi Jie Kim, Soo Yeun Lee, Seung Min Oh, Kyu Hyuck Chung, Genotoxic effects of silver nanoparticles stimulated by oxidative stress in human normal bronchial epithelial (BEAS-2B) cells Mutation Research/Genetic Toxicology and Environmental Mutagenesis. ,vol. 726, pp. 129- 135 ,(2011) , 10.1016/J.MRGENTOX.2011.08.008
Yadollah Omidi, Jaleh Barar, Hamid Reza Heidari, Somaieh Ahmadian, Hossein Ahmadpour Yazdi, Saghir Akhtar, Microarray analysis of the toxicogenomics and the genotoxic potential of a cationic lipid-based gene delivery nanosystem in human alveolar epithelial A549 cells Toxicology Mechanisms and Methods. ,vol. 18, pp. 369- 378 ,(2008) , 10.1080/15376510801891286