Proviral load and expression of avian leukosis viruses of subgroup C in long-term persistently infected heterologous hosts (ducks).

摘要: Proviral DNA load and expression of avian leukosis viruses subgroup C (ALV-C) in ducks infected mid embryogenesis were studied using quantitative PCR, RT-PCR, situ hybridization employing ALV-specific riboprobe, immunohistochemistry. A group long-term surviving, non-reviremic was selected for the study compared to control reviremic animals order obtain information about persisting retroviruses different duck tissues. widespread distribution proviruses tested tissues found, but proviral significantly lower comparison animals. The only exception brain blood cells, which no significant difference quantity integrated found between both categories ducks, thus indicating an exceptional position cells among all Contrary reviremic, not transcribed with thymus. In majority viral RNA revealed brain, infectious virus could be recovered from this tissue. opposite situation observed thymus, where remained below detection limit assay. As by analysis, either disseminated or focally distributed From follow up ALV-C intraembryonally we conclude that model is suitable retrovirus persistence accompained presence absence reviremias. possible consequences transmission heterologous host retroviral evolution are discussed.