A novel amperometric phenol biosensor based on immobilized HRP on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles for the determination of phenol derivatives
作者： Emre Çevik , Mehmet Şenel , Abdulhadi Baykal , M. Fatih Abasıyanık
DOI: 10.1016/J.SNB.2012.07.026
关键词:
摘要: Abstract A new type of amperometric phenol biosensor was fabricated for the determination and its derivatives such as, phenol, catechol, p -cresol, 2-aminophenol pyrogallol. The telomere poly(glycidylmethacrylate) (PGMA) with a trimethoxysilyl terminal group synthesized by telomerization glycidylmethacrylate. Iron oxide nanoparticles were coated in order to obtain good enzyme immobilization platform. PGMA covered Fe 3 O 4 covalently attached Au electrode surface horseradish peroxidase (HRP) immobilized on surface, respectively. telomere, modified electrodes characterized Fourier transform infrared (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM) cyclic voltammetry (CV) before after HRP. Amperometric response measured as function substrate concentration, at fixed potential −0.50 V vs. Ag/AgCl phosphate-buffered saline (pH 7.5). Analytical parameters obtained from calibration curves. basic features (optimum pH, optimum temperature, reusability, storage stability) determined.
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sci-hub.se 参考文章(53)
Mehmet Şenel, M. Fatih Abasıyanık, Construction of a Novel Glucose Biosensor Based on Covalent Immobilization of Glucose Oxidase on Poly(glycidyl methacrylate‐co‐vinylferrocene) Electroanalysis. ,vol. 22, pp. 1765- 1771 ,(2010) , 10.1002/ELAN.200900644
Z. Durmus, H. Kavas, A. Baykal, H. Sozeri, L. Alpsoy, S.Ü. Çelik, M.S. Toprak, Synthesis and characterization of L-carnosine coated iron oxide nanoparticles Journal of Alloys and Compounds. ,vol. 509, pp. 2555- 2561 ,(2011) , 10.1016/J.JALLCOM.2010.11.088
Xiaojun Chen, Jinwei Zhu, Zixuan Chen, Chenbin Xu, Yan Wang, Cheng Yao, A novel bienzyme glucose biosensor based on three-layer Au–Fe3O4@SiO2 magnetic nanocomposite Sensors and Actuators B-chemical. ,vol. 159, pp. 220- 228 ,(2011) , 10.1016/J.SNB.2011.06.076
Alan G. Heath, Water Pollution and Fish Physiology ,(1987)
Heiner. Kotte, Bernd. Gruendig, Klaus-Dieter. Vorlop, Beate. Strehlitz, Ullrich. Stottmeister, Methylphenazonium-modified enzyme sensor based on polymer thick films for subnanomolar detection of phenols Analytical Chemistry. ,vol. 67, pp. 65- 70 ,(1995) , 10.1021/AC00097A011
M.-C. Hennion, V. Pichon, D. Barceló, Surface water analysis (trace-organic contaminants) and EC regulations Trends in Analytical Chemistry. ,vol. 13, pp. 361- 372 ,(1994) , 10.1016/0165-9936(94)85007-0
Wenwen Zhao, Weimin Liu, Jiechao Ge, Jiasheng Wu, Wenjun Zhang, Xiangmin Meng, Pengfei Wang, A novel fluorogenic hybrid material for selective sensing of thiophenols Journal of Materials Chemistry. ,vol. 21, pp. 13561- 13568 ,(2011) , 10.1039/C1JM11768H
Songqin Liu, Jiuhong Yu, Huangxian Ju, Renewable phenol biosensor based on a tyrosinase-colloidal gold modified carbon paste electrode Journal of Electroanalytical Chemistry. ,vol. 540, pp. 61- 67 ,(2003) , 10.1016/S0022-0728(02)01276-7
ROMAN PIELASZEK, ANALYTICAL EXPRESSION FOR DIFFRACTION LINE PROFILE FOR POLYDISPERSIVE POWDERS Proceedings of the XIX Conference. pp. 43- 50 ,(2004) , 10.1142/9789812702913_0009
Richard A. Nyquist, Ronald O. Kagel, INFRARED SPECTRA OF INORGANIC COMPOUNDS Handbook of Infrared and Raman Spectra of Inorganic Compounds and Organic Salts#R##N#Infrared Spectra of Inorganic Compounds. pp. 1- 18 ,(1971) , 10.1016/B978-0-12-523450-4.50005-5