Isolation and Purification of a Polymeric Form of the Glycoprotein of Rabies Virus

摘要: Summary Of the three major proteins associated with rabies virus membrane, only glycoprotein was found to be located on external surface of membrane. Glycoprotein prepared by treatment Triton X-100 and purified isoelectric focusing homogeneous respect size point. This material, which is free phospholipids, able protect in vaccination experiments against a lethal challenge infection virus. The apparent mol. wt. this component isolated under non-denaturing conditions approx. 400000. same material analysed SDS polyacrylamide gel electrophoresis (PAGE) consist solely polypeptide chains G protein (mol. 80000). A minor (gp 50), detected PAGE released appeared breakdown product G-protein. Therefore detergent represents homopolymers Whether antigenic determinants reside monomeric subunit or are property polymeric form G-protein discussed.