A Counter Propagating Lens‐Mirror System for Ultrahigh Throughput Single Droplet Detection

摘要: Fluorescence-based detection schemes provide for multiparameter analysis in a broad range of applications the chemical and biological sciences. Toward realization fully portable systems, microfluidic devices integrating diverse functional components have been implemented out-of-lab environments. That said, there still exits an unmet recognized need miniaturized, low-cost, sensitive optical which not only efficient molecular excitation, but also enhanced photon collection capabilities. To this end, optofluidic platform that is adept at enhancing fluorescence light from channels presented. The central component module monolithic parabolic mirror located directly above channel, acts to enhance number emitted photons reflected toward detector. In addition, two-photon polymerization used print microscale-lens below flow channel opposite mirror, delivery excitation radiation into channel. Using such approach, it demonstrated signals can be by over two orders magnitude, with parallelization enabling pL-volume droplets rates up 40 000 per second.