Analysis of mouse brain microvascular endothelium using immuno‐laser capture microdissection coupled to a hybrid linear ion trap with Fourier transform‐mass spectrometry proteomics platform
作者： Qiaozhen Lu , Nivetha Murugesan , Jennifer A. Macdonald , Shiaw‐Lin Wu , Joel S. Pachter
关键词:
摘要: The purpose of this study was to identify the protein profile mouse brain microvascular endothelium in situ. This involved coupling a double-label, immuno-laser capture microdissection (LCM) process with LTQ-FT mass spectrometry perform situ proteomic analysis. LCM utilized isolate cells from frozen tissue sections. Following cell capture, samples were solubilized and proteins separated by gel electrophoresis preparation for enzymatic digestion LC-MS Processed subsequently analyzed using linear IT coupled Fourier transform spectrometer (LTQ-FT MS). Overall, study, 881 identified specific category immuno-guided probe these types along entirety cerebral tree. identification sufficient numbers high biological interest should allow us expression - as defined certain markers complex tissues.
core.ac.uk
sci-hub.se 参考文章(32)
Candace Graff, Gary Pollack, (Section B: Integrated Function of Drug Transporters In Vivo) Drug Transport at the Blood-Brain Barrier and the Choroid Plexus Current Drug Metabolism. ,vol. 5, pp. 95- 108 ,(2004) , 10.2174/1389200043489126
Panya S. Manoonkitiwongsa, Ernest F. Whitter, Wanpen Wareesangtip, Paul J. McMillan, Pedro B. Nava, Robert L. Schultz, Calcium-dependent ATPase unlike ecto-ATPase is located primarily on the luminal surface of brain endothelial cells. Histochemical Journal. ,vol. 32, pp. 313- 324 ,(2000) , 10.1023/A:1004093113985
D Miceli, R Ward, R L Collins, G Tanguay, A quantitative study of cerebrovascular variation in inbred mice. Journal of Anatomy. ,vol. 173, pp. 87- 95 ,(1990)
Kazuhiro Tetsuka, Hitomi Takanaga, Sumio Ohtsuki, Ken-ichi Hosoya, Tetsuya Terasaki, The l-isomer-selective transport of aspartic acid is mediated by ASCT2 at the blood-brain barrier. Journal of Neurochemistry. ,vol. 87, pp. 891- 901 ,(2004) , 10.1046/J.1471-4159.2003.02063.X
Jian Yi Li, Ruben J. Boado, William M. Pardridge, Blood—Brain Barrier Genomics Journal of Cerebral Blood Flow and Metabolism. ,vol. 21, pp. 61- 68 ,(2001) , 10.1097/00004647-200101000-00008
Jian Yi Li, Ruben J. Boado, William M. Pardridge, Rat Blood—Brain Barrier Genomics. II Journal of Cerebral Blood Flow and Metabolism. ,vol. 22, pp. 1319- 1326 ,(2002) , 10.1097/01.WCB.0000040944.89393.0F
Graeme I. Murray, An overview of laser microdissection technologies Acta Histochemica. ,vol. 109, pp. 171- 176 ,(2007) , 10.1016/J.ACTHIS.2007.02.001
Jeffrey K Harrison, Defang Luo, Wolfgang J Streit, In situ hybridization analysis of chemokines and chemokine receptors in the central nervous system Methods. ,vol. 29, pp. 312- 318 ,(2003) , 10.1016/S1046-2023(02)00354-7
Carine Fillebeen, Bénédicte Dehouck, Monique Benaïssa, Isabelle Dhennin-Duthille, Roméo Cecchelli, Annick Pierce, Tumor Necrosis Factor-α Increases Lactoferrin Transcytosis Through the Blood-Brain Barrier Journal of Neurochemistry. ,vol. 73, pp. 2491- 2500 ,(2002) , 10.1046/J.1471-4159.1999.0732491.X
Katie Kinnecom, Joel S. Pachter, Selective capture of endothelial and perivascular cells from brain microvessels using laser capture microdissection. Brain Research Protocols. ,vol. 16, pp. 1- 9 ,(2005) , 10.1016/J.BRAINRESPROT.2005.08.002