Detection of activated platelet-derived growth factor receptors in human meningioma

摘要: The beta receptor subunit of platelet-derived growth factor (PDGF) and its corresponding ligand (PDGF-BB) are coordinately expressed in fresh surgical isolates human meningioma. These observations imply that PDGF autocrine loops engaged meningioma suggest activated PDGF-beta receptors might contribute to the pathology this common brain neoplasm. study has been slowed by scarcity cell culture model systems. Furthermore, tumor tissue, activation state is difficult assess with conventional reagents, because intermixed normal stroma. In fact, there no evidence within ligand. We used a synthetic tyrosine phosphopeptide raise an antibody reports phosphorylation 751 receptor. Phosphorylated recognition site for phosphatidylinositol 3'-kinase, cytoplasmic effector PDGF-induced mitogenesis, chemotaxis, membrane ruffling. Immunoblotting immunostaining analyses show constitutively phosphorylated at multiple findings consistent role proliferation meningiomas.