Platelet-derived Growth Factor and Its Receptors in Human Glioma Tissue: Expression of Messenger RNA and Protein Suggests the Presence of Autocrine and Paracrine Loops

摘要: The expression of platelet-derived growth factor (PDGF) and its receptors was analyzed in 14 gliomas various degrees malignancy compared with three gliosis cases by situ hybridization immunohistochemistry techniques. Expression both PDGF A- B-chains higher glioblastomas than astrocytomas. A-chain mRNA predominantly found cell-rich areas glioblastomas. cognate PDGF-α receptor (PDGFR-α) heterogeneously distributed all grades, PDGFR-α gliosis. Within some probed complementary RNA, cells heavily loaded grains were intermingled others containing low or moderate signals. labeled often the vicinity proliferating capillaries. Immunostaining an anti-PDGF antibody affinity-purified antiserum against showed strong staining most tumor antibodies glioblastoma. In addition, yielded a scattered cells, few within astrocytoma. Furthermore, high levels PDGF-β (PDGFR-β) B-chain as well β protein hyperplastic These results suggest presence autocrine paracrine loops glioma, activating glioma PDGFR-β endothelial cells.