Receptor Mediated Delivery of Daunomycin Using Immunoliposomes: Pharmacokinetics and Tissue Distribution in the Rat

摘要: Pharmacokinetics and tissue distribution of daunomycin different liposomal formulations were determined. Special emphasis was thereby given to immunoliposome-mediated drug delivery. Three types 85 nm liposomes used for this study: 1) conventional liposomes, 2) sterically stabilized with 2000 Dalton polyethylene glycol 3) immunoliposomes prepared by coupling a control IgG2a or monoclonal antibody the distal end spacer. The OX26 rat transferrin receptor. Daunomycin administered i.v. injection rat. in rapidly cleared from plasma compartment. When compared free drug, did accumulate higher levels liver spleen lower heart, lung liver. In contrast, could not be detected lung, kidney, spleen. Using nonspecific isotype immunoliposomes, concentrations reduced at least factor two. Attachment more than 29 antibodies per liposome increase kidney lung. Tissue all organs coinjection unbound OX26. vitro, endocytosis fluorescent RG2 glioma cells observed. These data indicate that receptor mediated delivery tissues can achieved using conjugated immunoliposomes.