Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

摘要: The simplicity of homogeneous immunoassays makes them suitable for diagnostics acute conditions. Indeed, the absence washing steps reduces binding reaction duration and favors a rapid compact device, critical asset patients experiencing life-threatening diseases. In order to maximize analytical performance, standard systems employed in clinical laboratories rely largely on use high surface-to-volume ratio suspended moieties, such as microbeads, which provide at same time fast efficient collection analytes from sample controlled aggregation collected material improved readout. Here, we introduce an integrated microfluidic system that can perform analyte detection antibody-decorated beads their accumulation confined regions within 15 min. We concomitant analysis concentrations Neutrophil Gelatinase-Associated Lipocalin (NGAL) Cystatin C serum, two kidney injury (AKI) biomarkers. To this end, high-aspect-ratio, three-dimensional electrodes were channel impart trajectory microbeads through application dielectrophoretic (DEP) forces. Beads efficiently retained against fluid flow reagents, granting on-chip analyte-to-bead binding. Electrokinetic forces specific beads' size generated channel, leading differently decorated different readout chip. Therefore, multianalyte immunoassay was demonstrated powerful tool