CK2α phosphorylates DBC1 and is involved in the progression of gastric carcinoma and predicts poor survival of gastric carcinoma patients.

摘要: CK2a has diverse effects on the tumorigenesis owing to its kinase activity, which phosphorylates various proteins involved in tumorigenesis. We, therefore, investigated expression and role of phosphorylation deleted breast cancer 1 (DBC1) gastric carcinomas. We used 187 carcinomas human cells investigate roles relationship between DBC1 Positive phospho-DBC1 predicted shorter overall survival relapse-free by univariate analysis. Especially, was an independent prognostic indicator for carcinoma patients. In cells, bound phosphorylated DBC1. The evidenced co-immunoprecipitation a GST pull-down assay, vitro immunofluorescence staining. Inhibition both decreased proliferation invasive activity cells. Decreased migration associated with downregulation MMP2, MMP9 epithelial–mesenchymal transition. A mutation at site also downregulated signals related Our study demonstrated that is patients regulating addition, blocking inhibited potential Therefore, our suggests CK2a–DBC1 pathway might be new therapeutic target treatment carcinoma.