[62] Photosynthetic pyridine nucleotide reductase:

摘要: Publisher Summary This chapter describes the preparation and purification of photosynthetic pyridine nucleotide reductase. Enzymatic activity is determined by measuring initial rate formation TPNH in Hill reaction. In this procedure, photochemical reduction process followed directly increase optical density at 340 mμ. It noted that enzyme without effect on photolytic chloroplasts or c when measured spectrophotometrically with 2,3,6-trichlorophenolindophenol manometrically ferricyanide. The irreversibly photoinactivated presence chloroplasts. photoinactivation prevented a number reducing compounds such as cysteine, thioglycolate, glutathione, Under acid conditions, bleached, completely lost. appears hydrogen sulfide released from under these conditions. purified devoid ATPase activity, alcohol dehydrogenase either TPN DPN, lipoic DPN TPN.