Subcellular Distribution and Thermally-Induced Transition of Adenylate Cyclase Activity in Thermotolerant Tetrahymena Surface Membranes
作者： Hiroyuki Shimonaka , Yoshinori Nozawa
DOI: 10.1247/CSF.2.81
关键词:
摘要: The subcellular localization and thermally-induced transition of adenylate cyclase activity were studied in a thermotolerant strain Tetra-hymena pyriformis. A close relationship was present between the cell growth cycle. Maximum found early stationary phase cells. Adenylate primarily associated with surface membrane, pellicle, pellicle membrane measured as function temperature. Arrhenius plots its to be triphasic. marked change occurred at about 28°C small discontinuities around 15°C. Freeze-cleavage electron microscopic observations membranes revealed that membrane-intercalated particles began form aggregation clusters decreasing temperatures, below point (28°C) remarkable separation formed. These results from enzymatic morphological studies suggest may reflect altered physical states lipid caused by temperature changes.
sci-hub.se 参考文章(29)
Freeze-fracture studies of various membrane components isolated from Tetrahymena pyriformis cells. Journal of Electron Microscopy. ,vol. 24, pp. 155- 165 ,(1975) , 10.1093/OXFORDJOURNALS.JMICRO.A049968
Y. Nozawa, Chapter 6 Isolation of Subcellular Membrane Components from Tetrabymena Methods in Cell Biology. ,vol. 10, pp. 105- 133 ,(1975) , 10.1016/S0091-679X(08)60732-3
Y. Nozawa, H. Iida, H. Fukushima, K. Ohki, S. Ohnishi, Studies on Tetrahymena membranes: temperature-induced alterations in fatty acid composition of various membrane fractions in Tetrahymena pyriformis and its effect on membrane fluidity as inferred by spin-label study Biochimica et Biophysica Acta. ,vol. 367, pp. 134- 147 ,(1974) , 10.1016/0005-2736(74)90038-8
R.P. Brivio-Haugland, S.L. Louis, K. Musch, N. Waldeck, M.A. Williams, Liver plasma membranes from essential fatty acid-deficient rats. Isolation, fatty acid composition, and activities of 5'-nucleotidase, ATPase and adenylate cyclase. Biochimica et Biophysica Acta. ,vol. 433, pp. 150- 163 ,(1976) , 10.1016/0005-2736(76)90184-X
Y. Nozawa, H. Fukushima, H. Iida, Studies on Tetrahymena membranes: Modification of surface membrane lipids by replacement of tetrahymanol by exogenous ergosterol in Tetrahymena pyriformis Biochimica et Biophysica Acta. ,vol. 406, pp. 248- 263 ,(1975) , 10.1016/0005-2736(75)90008-5
F. Wunderlich, V. Speth, W. Batz, H. Kleinig, Membranes of Tetrahymena Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 298, pp. 39- 49 ,(1973) , 10.1016/0005-2736(73)90007-2
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Ira Pastan, George S. Johnson, Cyclic AMP and the transformation of fibroblasts. Advances in Cancer Research. ,vol. 19, pp. 303- 329 ,(1974) , 10.1016/S0065-230X(08)60057-3
J.R. Dickinson, M.G. Graves, B.E.P. Swoboda, Cyclic AMP metabolism in the cell cycle of tetrahymena pyriformis FEBS Letters. ,vol. 65, pp. 152- 154 ,(1976) , 10.1016/0014-5793(76)80468-1
P. W. Kreiner, J. J. Keirns, M. W. Bitensky, A temperature-sensitive change in the energy of activation of hormone-stimulated hepatic adenylyl cyclase. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 1785- 1789 ,(1973) , 10.1073/PNAS.70.6.1785