A dual role of EphB1/ephrin-B3 reverse signaling on migrating striatal and cortical neurons originating in the preoptic area: should I stay or go away?

摘要: During embryonic development the preoptic area (POA) gives rise to two populations of neurons which are generated at same time, cortical interneurons and striatal cells. POA-derived take a superficial path avoid developing striatum (Str) when they migrate their target region. We found that EphB1, is expressed in anlage, prevents from entering Str via ephrin-B3 reverse signaling. In contrast, for also express ephrin-B3, EphB1 acts as stop signal. This dual role due differences signaling cascades. For neurons, binding reduces endogenously high levels pSrc pFAK, then causes cells migration. EphB1-ephrin-B3 leads phosphorylation Src focal adhesion kinase (FAK) mediates repulsion. Consistent with these vitro findings, an knockout mouse line, we discovered misrouted over-migration Thus, EphB1/ephrin-B3 has different impact on sets time place: it can act repulsive cue migrating or terminate neuronal migration, novel Eph/ephrin system.