VH mutation status, CD38 expression level, genomic aberrations, and survival in chronic lymphocytic leukemia

摘要: In chronic lymphocytic leukemia (CLL), biologic risk factors such as immunoglobulin variable heavy chain gene (V(H)) mutation status, CD38 expression level, and genomic aberrations have recently been identified, but the relative prognostic impact of individual parameters is unknown. current study, we analyzed V(H) status by polymerase reaction sequencing (n = 300), fluorescence in situ hybridization (+3q, 6q-, +8q, 11q-, +12q, 13q-, t(14q), 17p-) triple-color FACS (CD5, CD19, CD38) 157) a unicentric CLL cohort. The influence rate level was tested maximally selected log-rank statistics. A corrected P value (P(cor)) for cutoff allowing best separation 2 subgroups with different survival probabilities identified at 97% homology (95% confidence interval [CI], 96%-98% homology, P(cor) <.001) 7% CI, 20%-71% expression, =.02). univariate analyses, unmutated genes high levels predicted shorter times. overall incidence similar mutated subgroups. High-risk 17p- 11q- occurred almost exclusively subgroup, whereas favorable 13q- single abnormalities were overrepresented subgroup. multivariate analysis, V(H), 17p deletion, 11q age, WBC, LDH independent factors, indicating complementary role to predict outcome CLL.