Gender difference in DNA adduct levels among nonsmoking lung cancer patients.

摘要: Lung cancer is the leading cause of mortality in Taiwanese women. Cigarette smoking cannot explain high lung this population because less than 10% women Taiwan are smokers. Therefore, environmental factors other may play an important role development female nonsmokers. The purpose study was to elucidate carcinogen exposure nonsmokers, based on DNA adduct formation. We collected nontumorous tissues resected from 62 nonsmoking patients and 20 noncancer controls investigate whether differences susceptibility formation exist between men (32)P-postlabeling ELISA (enzyme-linked immunosorbent assay) with polyclonal antibody against BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene)-DNA were used evaluate levels subjects. Our data showed that determined by both assays significantly higher those (P = 0.0001 for (32)P-postlabeling; P 0.01 ELISA). Moreover, females markedly greater males 0.014 0.001 difference could not be explained genetic polymorphisms cytochrome P-4501A1 (CYP1A1) or glutathione S-transferase (GSTM1), as polymerase chain reaction restriction fragment length polymorphism. These results demonstrate have a damage controls. In addition, derived observed male conclusion, partially rate