Pseudomonas spp. increases root biomass and tropane alkaloid yields in transgenic hairy roots of Datura spp.
作者： A. Moussous , C. Paris , M. Khelifi-Slaoui , M. Bekhouche , D. Zaoui
DOI: 10.1007/S11627-017-9862-1
关键词:
摘要: Transgenic hairy roots of Datura spp., established using strain A4 Agrobacterium rhizogenes, are genetically stable and produce high levels tropane alkaloids. To increase biomass alkaloid content this plant tissue, four Pseudomonas strains, fluorescens P64, P66, C7R12, putida PP01 were assayed as biotic elicitors on transgenic stramonium, tatula, innoxia. Alkaloids extracted from dried biomass, hyoscyamine scopolamine quantified liquid chromatography-tandem mass spectrometry analysis. D. stramonium innoxia production was stimulated by all spp. strains after a 5-d treatment. All P. increased yields compared to untreated cultures both 5 10 d Hyoscyamine highest in tatula exposed treatment with C7R12 (16.633 + 0.456 mg g−1 dry weight, 431% increase) although the yield increases control observed P64 (511% (583% for 10 d. showed elicitation 5 d (0.653 + 0.021 mg 265% P66 (5 d, 0.754 + 0.0.031 mg 321% increase; 0.634 + 0.046 mg 277% increase). These results show that studied here can positively significantly affect three species.
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