Immobilization of Fungal β-Glucosidase on Silica Gel and Kaolin Carriers

摘要: β-Glucosidase is a key enzyme in the hydrolysis of cellulose for producing feedstock glucose various industrial processes. Reuse through immobilization can significantly improve economic characteristics process. Immobilization fungal β-glucosidase by covalent binding and physical adsorption on silica gel kaolin was conducted consequent application these procedures large-scale Different parameters (incubation time, ionic strength, pH, enzyme/support ratio, glutaric aldehyde concentration, etc.) were evaluated their effect thermal stability immobilized enzyme. It shown that activity stable at 50 °C over 8 days. has also been case kaolin, approximately 95% initial onto support, loss not observed. However, to brings significant activity, only 35% preserved. In gradual desorption takes place. To prevent this process, we have carried out chemical modification protein. As result, after repeated washings, from reduced 75 20–25% loss.